{"data":{"id":89,"name":"Everted Intestinal Sac Method","abbreviation":"Everted_Sac","description":"

The everted intestinal sac (or ring) technique, introduced by Wilson and Wiseman (1954), is a classical ex vivo<\/em> model to study intestinal permeability and absorption. In this method, a segment of intestine is excised, everted to expose the mucosal surface outward, filled with buffer, and incubated in a solution containing the test compound. Transport into the serosal compartment reflects uptake across the intestinal wall.<\/p>

This assay preserves epithelial morphology and enzymatic activity, enabling assessment of both passive diffusion and active, carrier-mediated transport. It is inexpensive and allows regional studies along the length of the intestine. Limitations include short tissue viability and the absence of systemic circulation. Despite these, it remains a valuable tool for studies of drug absorption and nutrient bioavailability.<\/p>

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Source of image: 10.3390\/gidisord6010023<\/a><\/p>

<\/p>","categories":[{"id":168,"title":"Permeability","breadcrumb":[{"id":4,"title":"Experimental"},{"id":168,"title":"Permeability"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/methods\/89","landing_page":"https:\/\/molmedb.upol.cz\/method\/89","created_at":"2023-06-23T09:12:16.000000Z","updated_at":"2026-07-02T09:08:21.000000Z"}}