{"data":{"id":75,"name":"Electron paramagnetic resonance","abbreviation":"EPR","description":"<p>Electron paramagnetic resonance (EPR) is a biophysical technique that detects unpaired electrons in paramagnetic species, such as nitroxide radicals, to probe molecular microenvironments. Stable nitroxide spin probes (e.g., TEMPO) allow investigation of polarity, viscosity, and dynamics within biological and artificial membranes. In skin research, EPR enables direct assessment of stratum corneum lipid organization, hydration, and thickness by tracking partitioning of spin labels into hydrophilic and lipophilic domains. Compared with conventional permeability assays, EPR provides mechanistic insight into membrane heterogeneity and solute\u2013lipid interactions at high spatial resolution. Its non-destructive nature and sensitivity to local structural changes make EPR a valuable complementary tool for studying barrier function and drug or probe localization in membranes.<\/p>","categories":[{"id":7,"title":"Partitioning","breadcrumb":[{"id":4,"title":"Experimental"},{"id":7,"title":"Partitioning"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/methods\/75","landing_page":"https:\/\/molmedb.upol.cz\/method\/75","created_at":"2020-11-11T14:35:02.000000Z","updated_at":"2026-06-29T13:57:20.000000Z"}}