{"data":{"id":51,"name":"Perfusion Methods","abbreviation":"Perfusion","description":"<p>Perfusion methods are <em>in situ<\/em> techniques used to evaluate intestinal drug permeability under physiological conditions while preserving the intestinal blood supply and tissue integrity. They are applied during pharmaceutical development to predict intestinal absorption, estimate the fraction of an orally administered dose absorbed, and support Biopharmaceutics Classification System (BCS) permeability classification. Rat perfusion models have shown a good correlation with human intestinal permeability, making them valuable preclinical tools.<\/p><p>The two most commonly used approaches are <strong>single-pass intestinal perfusion (SPIP)<\/strong> and the <strong>intestinal closed-loop (ICL or Doluisio) method<\/strong>. In SPIP, a drug solution is continuously perfused through a defined intestinal segment, and permeability is calculated from the difference between inlet and outlet drug concentrations. In the closed-loop method, the drug solution remains within the isolated intestinal segment, and permeability is determined from the decrease in drug concentration over time. Both methods preserve physiological transport processes and allow investigation of regional permeability, transporter-mediated absorption, and the effects of formulation components or permeation enhancers.<\/p>","categories":[{"id":168,"title":"Permeability","breadcrumb":[{"id":4,"title":"Experimental"},{"id":168,"title":"Permeability"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/methods\/51","landing_page":"https:\/\/molmedb.upol.cz\/method\/51","created_at":"2019-08-05T14:49:19.000000Z","updated_at":"2026-07-03T07:51:34.000000Z"}}