{"data":{"id":44,"name":"Parallax fluorescence quenching","abbreviation":"Parallax","description":"<p>The <strong>parallax method<\/strong> is a fluorescence quenching technique used to determine the depth of fluorophores or specific sites of proteins and lipids within lipid bilayers. It relies on the use of spin-labeled phospholipids carrying nitroxide groups at defined positions along the acyl chains or in the polar headgroup. By comparing the extent of fluorescence quenching produced by two spin-labels located at different depths, the vertical position of the fluorophore relative to the bilayer center can be calculated with angstrom precision. This method has been widely applied to localize tryptophan residues in proteins, fluorescent lipid analogues such as NBD-labeled phospholipids, and probes like anthroyloxy fatty acids. Extensions of the technique include quenching in the polar headgroup region and time-resolved studies of fluorophore relaxation.<\/p>","categories":[{"id":167,"title":"Positioning","breadcrumb":[{"id":4,"title":"Experimental"},{"id":167,"title":"Positioning"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/methods\/44","landing_page":"https:\/\/molmedb.upol.cz\/method\/44","created_at":"2019-05-06T09:56:44.000000Z","updated_at":"2026-07-08T08:23:56.000000Z"}}