{"data":{"id":35,"name":"ELFA","abbreviation":"ELFA","description":"<h2>Liposomal fluorescence assay<\/h2>\r\n\r\n<div style=\"text-align:justify\">This method is capable to determine permeation kinetics of basic drug-like solutes across lipid bilayers. The assay is based on the hypothesis that permeation of a weak base along a concentration gradient results in net proton release at the cis-side and net proton capture at the trans-side of the bilayer. Proton release by the basic small molecule occurs prior to membrane permeation, and proton uptake occurs after permeation. The resulting pH changes are monitored with pH-sensitive fluorophores.<\/div>\r\n","categories":[{"id":168,"title":"Permeability","breadcrumb":[{"id":4,"title":"Experimental"},{"id":168,"title":"Permeability"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/methods\/35","landing_page":"https:\/\/molmedb.upol.cz\/method\/35","created_at":"2018-11-13T12:54:35.000000Z","updated_at":"2019-07-19T07:10:29.000000Z"}}