A hexadecane liquid layer is widely used as an artificial membrane to model passive transcellular diffusion in permeability assays (e.g. HDM-PAMPA). A thin (\u22489\u201310 \u00b5m) hexadecane layer separates donor and acceptor compartments, providing a reproducible hydrophobic barrier. It mimics the hydrocarbon core of phospholipid bilayers, which represents the principal barrier to passive diffusion in biological membranes.<\/p>
Permeability values obtained correlate well with human gastrointestinal absorption and offer better predictive power than simple partition coefficients. The system also enables pH-dependent profiling and correction for unstirred water layer effects. While it does not capture active transport or efflux mechanisms, the hexadecane membrane provides a simple, high-throughput, and robust tool for ranking compounds by passive permeability in early drug discovery.<\/p>
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<\/p>","categories":[{"id":65,"title":"Model","breadcrumb":[{"id":58,"title":"Generic"},{"id":65,"title":"Model"}]}],"url":"https:\/\/molmedb.upol.cz\/api\/v1\/membranes\/37","landing_page":"https:\/\/molmedb.upol.cz\/membrane\/37","created_at":"2019-08-21T07:09:14.000000Z","updated_at":"2026-06-29T08:23:40.000000Z"}}